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View DAD/PDA 3D Matrix

The ChromSpec menu contains the View DAD/PDA 3D Matrix... option for a full resolution contour display of all data in the matrix. It is a fast rendering of all of the data in the DAD/PDA matrix, and should not be used for the D1 zeroing of components. For the determination of the D1 wavelengths, the Reduce DAD/PDA 3D Matrix.. should be used.

The Logarithmic Z graph button in the View DAD/PDA 3D Matrix plots offers a third SymLog Z-scale option. With the RdBu_r colormap (or another that goes to white at 0), you can judge the efficacy of the background subtraction. Here we see the efficacy of the lsrpls on a UHPLC separation with a strongly varying gradient baseline. This is important for the Filter DAD/PDA Baseline option for determining the efficacy of the background filtering.

In this visualization, there is a symmetrix log scale that allows very close to 0 values to be plotted with light red or blue colors. It allows you to see the effectiveness of the Whittaker algroithms across all 611 wavelengths in this PDA matrix. This represent an excellent background subtraction.

If you look closely, you will see the many trace close-to-baseline components as well as the wavelengths where each of those components are signaled. The early and late eluting bands are the dead-time solvents and the final isocratic

This is the matrix for this gradient UHPLC separation without this background filtration step. The gradient will generally make it very difficult to visualize the close to baseline threshold components in the 3D contour.