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Chromatography Functions

HVL (Haarhoff-Van der Linde)

The HVL (full range) model:

HVL (FULL RANGE)5.PNG

a0 = Area

a1 = Center (as mean of asymmetric peak)

a2 = Width (SD of underlying Gaussian)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

 

Built in model: HVL

User-defined peaks and view functions: HVL(x,a0,a1,a2,a3)

v5_HVL.png

GenHVL - Default Generalized Normal ZDD

The GenHVL model:

GENHVL1.PNG

a0 = Area

a1 = Center (as mean of asymmetric peak)

a2 = Width (SD of underlying Gaussian ZDD)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = ZDD asymmetry ( -1 > a4 > 1 )

 

Built in model: GenHVL

User-defined peaks and view functions: GenHVL(x,a0,a1,a2,a3,a4)

 

v5_GenHVL.png

GenHVL[Z] - [Z] Generalized Normal ZDD

 

The GenHVL[Z] model:

GENHVL[Z]1.PNG

a0 = Area

a1 = Center (as mean of generalized normal ZDD)

a2 = Width (SD of underlying Gaussian)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = ZDD asymmetry ( -1 > a4 > 1 )

 

Built in model: GenHVL[Z]

User-defined peaks and view functions: GenHVL[Z](x,a0,a1,a2,a3,a4)

v5_GenHVL[Z].png

GenHVL[Y] - [Y] Generalized Error Model ZDD

The GenHVL[Y] model:

GENHVL[Y]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying normal ZDD)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = Power n in exp(-zn) decay ( .25 > a4 > 4 ) adjusts kurtosis (fourth moment)

a5 = ZDD asymmetry ( -1 > a5 > 1 ), adjusts skew (third moment)

 

Built in model: GenHVL[Y]

User-defined peaks and view functions: GenHVL[Y](x,a0,a1,a2,a3,a4,a5)

v5_GenHVL[Y].png

GenHVL[T] - [T] Generalized Student's t ZDD

The GenHVL[T] model:

GENHVL[T]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying normal ZDD)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = Student's t tailing, the nu or DOF ( 1 > a4 > 1,000,000 ) (fourth moment)

a5 = ZDD asymmetry ( -1 > a5 > 1 ), adjusts skew (third moment)

 

Built in model: GenHVL[T]

User-defined peaks and view functions: GenHVL[T](x,a0,a1,a2,a3,a4,a5)

v5_GenHVL[T].png

GenHVL[V] - [V] Generalized Error Model ZDD

The GenHVL[V] model:

GENHVL[V]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying normal ZDD)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = The GMG half-Gaussian convolution width, adjusts skew (third moment)

a5 = ZDD asymmetry ( -1 > a5 > 1 ), adjusts skew (third moment)

 

Built in model: GenHVL[V]

User-defined peaks and view functions: GenHVL[V](x,a0,a1,a2,a3,a4,a5)

v5_GenHVL[V].png

GenHVL[G] - [G] Half-Gaussian Modified Gaussian (GMG)

The GenHVL[G] model:

GENHVL[G]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying Gaussian)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = The GMG half-Gaussian convolution width

 

Built in model: GenHVL[G]

User-defined peaks and view functions: GenHVL[G](x,a0,a1,a2,a3,a4)

v5_GenHVL[G].png

GenHVL[E] - [E] Exponentially-Modified Gaussian (EMG)

The GenHVL[E] model:

GENHVL[E]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying Gaussian)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = The EMG half-Gaussian convolution width

 

Built in model: GenHVL[E]

User-defined peaks and view functions: GenHVL[E](x,a0,a1,a2,a3,a4)

v5_GenHVL[E].png

GenHVL[K] - [K] Generalized ZDD

The GenHVL[K] model:

GENHVL[K]2.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying normal ZDD)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = The fractional order of the decay from the apex of the peak upward

a5 = The fractional order of the rise to the apex

 

Built in model: GenHVL[K]

User-defined peaks and view functions: GenHVL[K](x,a0,a1,a2,a3,a4,a5)

v5_GenHVL[K].png

GenHVL[Q] - [Q] Error Model (Symmetric)

The GenHVL[Q] model:

GENHVL[Q]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying Gaussian)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = ZDD power of tailing ( 0.25 > a4 > 4 )

 

Built in model: GenHVL[Q]

User-defined peaks and view functions: GenHVL[Q](x,a0,a1,a2,a3,a4)

v5_GenHVL[Q].png

GenHVL[S] - [S] Student's t (Symmetric)

The GenHVL[S] model:

GENHVL[S]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying Gaussian)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = Student's t tailing, the nu or DOF ( 1 > a4 > 1,000,000 )

 

Built in model: GenHVL[S]

User-defined peaks and view functions: GenHVL[S](x,a0,a1,a2,a3,a4)

v5_GenHVL[S].png

GenHVL[YpE] - [YpE] Generalized Laplace ZDD

The GenHVL[YpE] model:

GENHVL[D]1.PNG

a0 = Area

a1 = Center (as mean of underlying normal ZDD)

a2 = Width (SD of underlying normal ZDD)

a3 = HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = ZDD asymmetry ( -1 > a4 > 1 ), adjusts skew (third moment)
 

Built in model: GenHVL[YpE]

User-defined peaks and view functions: GenHVL[YpE](x,a0,a1,a2,a3,a4)

v5_GenHVL[D].png

 

GenHVL[Yp] - [Yp] Generalized Error Model ZDD

The GenHVL[Yp] model model is identical to GenHVL[Y] model, but uses a separate starting estimate algorithm which assumes high overload preparatory peak shapes.

 

NLC (Wade-Thomas Non-Linear Chromatography)

The NLC (full range) model:

7NLC (FULL RANGE)9.PNG

a0 = Area

a1 = Center (as mean of asymmetric peak)

a2 = Width (NLC/Giddings kinetic time constant)

a3 = NLC chromatographic distortion ( -1 > a3 > 1 )

 

Built in model: NLC

User-defined peaks and view functions: NLC(x,a0,a1,a2,a3)

 v5_NLC.png

GenNLC[Z] - [Z] ZDD

The GenNLC[Z] is derived using the relationships of equivalence between the GenHVL and GenNLC models:

GENNLC[Z]8.PNG

To convert the GenHVL[Z] to the GenNLC[Z], the a2 is converted to a Giddings kinetic time constant, the a4 is converted to a Gidding's indexed asymmetry.

a0 = Area

a1 = Center (as mean of generalized normal ZDD)

a2 = Kinetic Width (Giddings time constant of ZDD)

a3 = NLC/HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = NLC indexed asymmetry ( -10 > a4 > 10 ) a4=0.5 NLC (Giddings)

 

Built in model: GenHVL[Z]

User-defined peaks and view functions: GenHVL[Z](x,a0,a1,a2,a3,a4)

v5_gennlc[z].png

GenNLC[Y] - [Y] ZDD

The GenNLC[Y] model is derived using the relationships of equivalence between the GenHVL and GenNLC models:

GENNLC[Y]3.PNG

To convert the GenHVL[Y] to the GenNLC[Y], the a2 is transformed to a Giddings kinetic time constant, the a5 is transformed to a Gidding's indexed asymmetry.

a0 = Area

a1 = Center (as mean of generalized normal ZDD)

a2 = Kinetic Width (Giddings time constant of ZDD)

a3 = NLC/HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = Power n in exp(-zn) decay ( .25 > a4 > 4 ), adjusts kurtosis (fourth moment)

a5 = NLC indexed asymmetry ( -10 > a5 > 10 ) a5=0.5 NLC (Giddings), adjusts skew (third moment)

 

Built in model: GenNLC[Y]

User-defined peaks and view functions: GenNLC[Y](x,a0,a1,a2,a3,a4,a5)

 

v5_GenNLC[Y].png

GenNLC[T] - [T] ZDD

The GenNLC[T] model is derived using the relationships of equivalence between the GenHVL and GenNLC models:

GENNLC[T]3.PNG

To convert the GenHVL[T] to the GenNLC[T], the a2 is transformed to a Giddings kinetic time constant, the a5 is transformed to a Gidding's indexed asymmetry.

a0 = Area

a1 = Center (as mean of generalized normal ZDD)

a2 = Kinetic Width (Giddings time constant of ZDD)

a3 = NLC/HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = Student's t tailing, the nu or DOF ( 1 > a4 > 1,000,000 ) (fourth moment)

a5 = NLC indexed asymmetry ( -10 > a5 > 10 ) a5=0.5 NLC (Giddings), adjusts skew (third moment)

 

Built in model: GenNLC[T]

User-defined peaks and view functions: GenNLC[T](x,a0,a1,a2,a3,a4,a5)

 

v5_GenNLC[T].png

GenNLC[V] - [V] ZDD

The GenNLC[V] model is derived using the relationships of equivalence between the GenHVL and GenNLC models:

GENNLC[V]2.PNG

To convert the GenHVL[V] to the GenNLC[V], the a2 is transformed to a Giddings kinetic time constant, the a5 is transformed to a Gidding's indexed asymmetry.

a0 = Area

a1 = Center (as mean of generalized normal ZDD)

a2 = Kinetic Width (Giddings time constant of ZDD)

a3 = NLC/HVL Chromatographic distortion ( -1 > a3 > 1 )

a4 = The GMG half-Gaussian convolution width, adjusts skew (third moment)

a5 = NLC indexed asymmetry ( -10 > a5 > 10 ) a5=0.5 NLC (Giddings), adjusts skew (third moment)

 

Built in model: GenHVL[V]

User-defined peaks and view functions: GenHVL[V](x,a0,a1,a2,a3,a4,a5)

 

v5_GenNLC[V].png

The Giddings Model

The Giddings model:

GIDDINGS1.PNG

a0 = Area

a1 = Center (as mean of asymmetric peak)

a2 = Kinetic Width (as time constant)

 

Built in model: Giddings

User-defined peaks and view functions: Giddings(x,a0,a1,a2)

v5_Giddings.png

EMG Exponentially Modified Gaussian

The EMG Model:

EMG1.PNG

a0 = Area

a1 = Center (as mean of Gaussian peak that is convolved by the a3 exponential)

a2 = Width (SD of the Gaussian peak that is convolved by the a3 exponential)

a3 = The first order kinetic or exponential decay width convolving the Gaussian (can be negative to model fronted peaks)

 

Built in model: EMG

User-defined peaks and view functions: EMG(x,a0,a1,a2,a3)

 

v5_EMG.png

GMG Half-Gaussian Modified Gaussian

The GMG Model:

 

GMG1.PNG

a0 = Area

a1 = Center (as mean of Gaussian peak that is convolved by the a3 half-Gaussian)

a2 = Width (SD of the Gaussian peak that is convolved by the a3 half-Gaussian)

a3 = The first order probabilisitic half-Gaussian width convolving the Gaussian (can be negative to model fronted peaks)

 

Built in model: GMG

User-defined peaks and view functions: GMG(x,a0,a1,a2,a3)

 

v5_GMG.png

Log-Normal 4-Parameter Generalized Normal

The LN4 Model:

LN4 GENERALIZED NORMAL1.PNG

a0 = Area

a1 = Center (mode of asymmetric peak)

a2 = Half-Height Width (FWHM)

a3 = Half-Height Asymmetry Ratio (Asym50)

 

Built in model: LN4 or GenNorm[cwa]

User-defined peaks and view functions: LN4(x,a0,a1,a2,a3) or GenNorm[cwa](x,a0,a1,a2,a3)

 

 

v5_LN4.png

 

 

 

 

 

 

 

 


PeakLab v1 Documentation Chromatography Notes HVL (Full Range)